Corn Genetics: Chi Square Analysis Data Sheet Chi Square Analysis of Corn Cross #1 What ratio is evident in the data? Mendelian Genetics and Chi Square Analysis This lab was written by David J. Grisé and R. Deborah Overath Pre lab assignment. Attach A Separate Sheet If Necessary. Change ). Your Tentative Hypothesis: The black eyed parents were Bb x Bb. Procedure: On two monohybrid corn, count the number of purple and yellow kernels and the smooth and shrunken kernels. Results #2: This is the second part of the lab with one dihybrid cross corn. Circle that Problem: A sample of mice (all from the same parents) shows 58 Black hair, black eyes | 16 Black hair, red eyes | 19 White hair, black eyes 7 | White hair, red eyes, Your tentative hypothesis: (what are the parents?) In your case, 4 - 1 = 3. Find Purple & smooth ___ Purple & shrunken ____ Yellow & smooth ___ Yellow & shrunken ____. Materials • Corn Genetics and Statistical Analysis PowerPoint (available at www.kscorn.com) • Dihybrid Corn Ears from Carolina Biological, R and Su Alleles 9:3:3:1 (item #176600) one for each group • Corn Genetics Student Handout (pg. The table below will help you make the calculations. 5. In this lab we observed the proportions of dominant and recessive phenotypes that resulted from different crosses and analyzed them using a chi square in order to learn about the inheritance of genes and how to use chi squares. These numbers are entered in Columns 1 and 2 of the . data are due to chance alone or if the data is significantly different, you Name:______________________________________________, Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. Hypothesis. (271-243.5625)^2/243.5625 +  (73-81.1875)^2/81.1875 + (63-81.1875)^2/81.1875 + (26-27.0625)^2/27.0625. Objective: To observe phenotypes and use chi square analysis to determine if results are of good fit. (PpSs x PpSs) involving two pairs of heterozygous genes resulting in a theoretical (expected) ratio of 9:3:3:1. Hypothesis: The (the second part of the lab) corn is a dihybrid cross of two monohybrids. The chart below goes through the chi square analysis. #1 So the corn has 433 grains, 271 Purple and Starchy, 73 purple and sweet, 63 yellow and starchy, and 26 yellow sweet. Corn can be purchased from biological supply companies or through Amazon. the number in that row that is closest to your chi square value. record the data and find the percentage. 4. need to use a chi square test. I used the formula of the observed value minus the expected value and all squared and divided by the expected value. In this exercise, you will ratio? CORN GENETICS & CHI SQUARE ANALYSIS In this exercise, you will examine an ear of corn and determine the type of cross and genes responsible for the coloration and texture of the corn kernels like the one show below. In other words, it also fits well with the hypothesis. To determine if the deviations from your observed Change ), You are commenting using your Google account. From my results, I got a total of 222 kernels. In this set, because only two values (traits) are examined, the degrees of freedom (df) is 1. Of course, there could be two reasons why data could have a poor chi square fit. It seems that the final value was closest to 1.42, which is at a 70% chance probability that the results happened through chance. Be sure you understand and can explain gene, allele, dominant allele, recessive allele, genotype, phenotype, homozygous, heterozygous and know how to use a Punnett square to cross two heterozygous individuals. Objective: To observe phenotypes and use chi square analysis to determine if results are of good fit. Problem: In a certain reptile, eyes can be either black or yellow. It seems that the results fit pretty well. 2. SHOW ALL WORK! We will now consider Corn Genetics Chi Square Analysis Last updated; Save as PDF Page ID 19919; Contributed by Shannan Muskopf; High School Biology Instructor at Granite City School District; Sourced from Biology Corner; Dihybrid Cross. First, find the expected numbers of each ratio. the rows above). This answer falls near the 50%. Now determine if your It could just be bad luck, or there is something happening with the gene. Create a punnett square or use a mathematical system to determine the phenotype ratio. parental generation was PpSs x PpSs? Using the ratio of contrasting phenotypic traits in an ear of corn, you will model genetic expression of traits such as kernel color and texture. Our chi square value was 0.849478749 means that it was a “good fit” and supports our hypothesis. Chi-square Analysis with Corn Genetics In this investigation, you will analyze the phenotypes of F2 generation ears of corn produced from monohybrid and dihybrid crosses. So, we will just go with the standard 9:3:3:1 ratio and formula observed value minus expected value all squared and divided by the expected value. Did you obtain a 9:3:3:1 Purple and smooth (A), Purple and The observed numbers I got was 131, 40, 40, and 11. Two black eyed lizards are crossed, and the result is 72 black eyed lizards, and 28 yellow-eyed lizards. All analysis question answers and a completed lab worksheet can be found in the file that is attached at the beginning of this blog lab report.

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